Paraffin Processing of Tissue Protocols Online

https://www.protocolsonline.com/histology/sample-preparation/paraffin-processing-of-tissue/ Oct 03, 2016 · Embedding tissues in paraffin blocks Tissues processed into paraffin will have wax in the cassettes; in order to create smooth wax blocks, the wax first needs to be melted away placing the entire cassette in 58°C paraffin bath for 15 minutes.…

Paraffin embedding and sectioning - OpenWetWare

https://openwetware.org/wiki/Paraffin_embedding_and_sectioning Paraffin embedding instruments are available. They dehydrate the sample to 100% ethanol and then infiltrate the tissue with xylene and later molten paraffin at 60-70ºC. Avoid paraffinisation temperatures above 65º if you want to label with antibodies later on, since epitopes can be destroyed.…

Paraffin Embedding - an overview ScienceDirect Topics

https://www.sciencedirect.com/topics/medicine-and-dentistry/paraffin-embedding Embedding tissues in agar prior to paraffin embedding is very beneficial when working with small and friable samples (Lund et al., 1961).It minimizes the loss of tissue during vigorous processing procedures, allows placement of multiple small tissues within one block, and provides orientation for sectioning.…

Tissue fixation, embedding and sectioning Abcam

https://www.abcam.com/kits/tissue-fixation-embedding-and-sectioning When generating paraffin-embedded tissue samples, the tissue must be fixed before embedding in paraffin. Fixation is achieved by perfusion or immersion immediately following dissection. The process typically takes 4 - 24 hours (fixation for longer than 24 hours is not recommended as it may lead to overfixation, which may mask the antigen).…

Sectioning of paraffin-embedded tissue protocol Abcam

https://www.abcam.com/protocols/sectioning-of-paraffin-embedded-tissue-video-protocol Embedding tissue into paraffin blocks supports the tissue structure and enables very thin sections to be cut and mounted onto microscope slides for analysis. This protocol describes how to cut sections from tissue embedded in paraffin blocks (2:48 minutes).…